Raji cells typically grow as floating clusters of various sizes in suspension. Clusters can get very large as the density of the culture increases although some single cells might be also present. Debris is normal with this cell line.
It is recommend to remove the DMSO when initiating the culture and follow the instructions on the ATCC product information sheet for thawing the vial. It is very important that Raji cells are maintained at the correct density in the ATCC-recommended complete growth medium. Check the Certificate of Analysis to determine the correct volume of medium to achieve an initial seeding density of 2-3 X 10^5 viable cells/mL.
Cell counts should be repeated every 2-3 days when feeding the cells. Cultures can be maintained by the simple addition of fresh medium. The cell concentration should not be allowed to exceed 2-3 x 10^6 cell/ml, sufficient medium should be added to bring the cell concentration down to 3-5 x 10^5 viable cells/ml and to avoid excessive acidification of the growth medium. If the medium is simply replaced (by spinning down and resuspending in the same volume) and the cell density is not decreased, the cells will deplete the medium and die. If the cells are diluted below their minimum density, they may either enter into a lag phase and grow very slowly, or they will die.
|Date Created||05/18/2018 12:58 PM
|Date Updated||05/18/2018 01:06 PM