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Milk in PCR reaction with ATCC 16404, Aspergillus brasiliensis.

Why is it recommended that milk be added to a PCR reaction using DNA from Aspergillus brasiliensis, ATCC 16404?

Amplifying the DNA from ATCC 16404  (and other black Aspergillus species) is often inhibited by polyphenolic compounds which are rich in dark pigmented molds.  Although the mechanism is not fully understood, it is known that adding a small amount of BLOTTO or non-fat milk cocktails to the PCR mix may help amplify those DNA by eliminating the effect of those inhibitory compounds  We use 1 ul of milk powder solution (50 mg/ml) in 25 ul of PCR mix. A reference for this is DeBoer SH, et al. Nucleic Acids Research 23: 2567-2568, 1995.

Date Created08/20/2015 04:49 PM
Date Updated08/20/2015 04:59 PM

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