Amplifying the DNA from ATCC 16404 (and other black Aspergillus species) is often inhibited by polyphenolic compounds which are rich in dark pigmented molds. Although the mechanism is not fully understood, it is known that adding a small amount of BLOTTO or non-fat milk cocktails to the PCR mix may help amplify those DNA by eliminating the effect of those inhibitory compounds We use 1 ul of milk powder solution (50 mg/ml) in 25 ul of PCR mix. A reference for this is DeBoer SH, et al. Nucleic Acids Research 23: 2567-2568, 1995.
|Date Created||08/20/2015 04:49 PM
|Date Updated||08/20/2015 04:59 PM