Broth propagation can not be used to grow ATCC® 15597-B1™. It is very important that dilutions of the phage must be spotted onto prepared plates, which contain ATCC® 15597™ only in the agar overlay. We observe plaques visibly formed from 10(2) to 10(9) dilutions after at least 24 hours incubation, using the recommended culture conditions. We recommend just a drop or two (10 ul) of the host be added to 2.5 mL of the top agar so that the host does not overgrow the phage. We do not recommend mixing the phage and E. coli together and pouring that on a plate, or growing the two together as a liquid culture. These two methods are not reliable and make it difficult to see any clearing.
|Date Created||12/11/2017 04:53 PM
|Date Updated||12/11/2017 04:53 PM