It is normal for these cells to be slow to recover from cryopreservation and they may take 7-14 days to begin actively growing. The viability may drop initially, unfortunately this is normal. CRL-1573.3 is a suspension line however there will be some cells that are semi-attached. There will be some single attached cells with small processes and some clusters, but these will detach easily with a gentle tap of the flask. We maintain these cells by simply adding fresh medium 2 to 3 times each week, diluting the culture back down to a cell concentration between 1 x 10(5) and 3 x 10(5) cells/mL. The culture should be transferred to larger flasks (or more flasks) as necessary.
|Date Created||12/18/2017 10:41 AM
|Date Updated||12/18/2017 10:42 AM