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Reovirus 3 (ATCC® VR-824)

Classification: Reoviridae, Orthoreovirus, Mammalian orthoreovirus  /  Product Format: frozen 1.0 mL

Classification Reoviridae, Orthoreovirus, Mammalian orthoreovirus
Deposited As Reovirus type 3
Agent Reovirus 3
Strain Dearing
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images VR-824 images
Comments
Hemagglutinates with RBC (Gomatos PJ, Tamm, I. Reactive sites of reovirus type 3 and their interaction with receptor substances. Virology 17:455-461, 1962. PubMed: 13900038).
Effect on Host
CPE, granulation and sloughing
Recommended Host
LLC-MK2 Derivative (ATCC® CCL-7.1™)
Growth Conditions Temperature: 36°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-5 days at 36°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, granulation and sloughing
Name of Depositor WA Chappell
Source Child with diarrhea
References

Ramos-Alvarez M, Sabin AB. Enteropathogenic viruses and bacteria; role in summer diarrheal diseases of infancy and early childhood. J. Am. Med. Assoc. 167: 147-156, 1958. PubMed: 13538681

Gomatos PJ, Tamm, I. Reactive sites of reovirus type 3 and their interaction with receptor substances. Virology 17:455-461, 1962. PubMed: 13900038

Cross References

Nucleotide (GenBank) : AJ133091 Reovirus type 3 S2 gene, strain Dearing.