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Quantitative Synthetic Human coronavirus NL63 RNA (ATCC® VR-3263SD)

Product Format: frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable

Permits and Restrictions

View Permits

Agent Quantitative Synthetic Human coronavirus NL63 RNA
Applications ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable
Storage Conditions -70°C or colder
Intended Use The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

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Comments

Manufactured under ISO 13485 guidance

Preparation includes fragments from NSP3 (ORF 1A), Rdrp (nsp12), NTPase (nsp13), nsp16, spike protein, nucleocapsid, and 3’ untranslated regions.

The following primers and probe can be used with this nucleic acid preparation:
Forward Primer (5’ to 3’): AGGACCTTAAATTCAGACAACGTTCT
Reverse Primer (5’ to 3’): GATTACGTTTGCGATTACCAAGACT
Probe (5’ to 3’): /56-FAM/TAACAGTTTTAGCACCTTCCTTAGCAACCCAAACA/36-TAMSp/

Name of Depositor ATCC
Special Collection RNA
References

Cui LJ, et al. Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China. Adv Virol 2011: 129134, 2011. PubMed: 22315599

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation