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Quantitative Synthetic Hepatitis E virus RNA (ATCC® VR-3258SD)

Product Format: frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable

Permits and Restrictions

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Agent Quantitative Synthetic Hepatitis E virus RNA
Common Name HEV
Applications ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable
Storage Conditions -70°C or colder
Intended Use The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

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Comments Manufactured under ISO 13485 guidance

Preparation includes fragments of the 5’ untranslated region, methyl transferase, Y domain, X domain, helicase, RNA-directed RNA polymerase, and open reading frames 2 and 3 (ORF2 and ORF3).


The following primers and probe can be use with this nucleic acid preparation RefBarnaud E, et al. Thermal inactivation of infectious hepatitis E virus in experimentally contaminated food. Appl Environ Microbiol 78(15): 5153-5159, 2012. PubMed: 22610436:
Forward primer: GGTGGTTTCTGGGGTGAC
Reverse primer: AGGGGTTGGTTGGATGAA
Probe: FAM-TGATTCTCAGCCCTTCGCAATCC-TAMRA
Name of Depositor ATCC
Special Collection RNA
References

Muñoz-Chimeno M, et al. Full coding hepatitis E virus genotype 3 genome amplification method. J Virol Met 230: 18-23, 2016.

Sauleda S, et al. Seroprevalence of hepatitis E virus (HEV) and detection of HEV RNA with a transcription-mediated amplification assay in blood donors from Catalonia (Spain). Transfusion 55(5): 972-979, 2015. PubMed: 25403913

Barnaud E, et al. Thermal inactivation of infectious hepatitis E virus in experimentally contaminated food. Appl Environ Microbiol 78(15): 5153-5159, 2012. PubMed: 22610436

Fu H, et al. Hepatitis E virus infection among animals and humans in Xinjiang, China: possibility of swine to human transmission of sporadic hepatitis E in an endemic area. Am J Trop Med Hyg 82(5): 961-966, 2010. PubMed: 20439982

Matsubayashi K, et al. A case of transfusion-transmitted hepatitis E caused by blood from a donor infected with hepatitis E virus via zoonotic food-borne route. Transfusion 48(7): 1368-1375, 2008. PubMed: 18651907

Ahn JM, et al. Comparison of real-time reverse transcriptase-polymerase chain reaction and nested or commercial reverse transcriptase-polymerase chain reaction for the detection of hepatitis E virus particle in human serum. Diagn Microbiol Infect Dis 56(3): 269-274, 2006. PubMed: 16757142

Meng XJ, et al. A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci USA 94(18): 9860-9865, 1997. PubMed: 9275216

Cross References

Nucleotide (GenBank) : X98292.1 Hepatitis E virus, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation