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BetI Repression Pair (TetR Homolog) (ATCC® SB-2011)

Permits and Restrictions

View Permits

Preparation
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Components
Synthetic TetR homolog regulator:
pENTR_L1L2_BetI (ATCC SB-1085)

Synthetic promoter:
pENTR_L4R1_pGPD-BetI (ATCC SB-1065)
Application To construct a gene expression system via a TetR homolog-based repression system
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

This pair of products includes two plasmids: pENTR_L1L2_ QacR contains a TetR homolog, QacR, (ATCC SB-1090) and pENTR_L4R1_pGPD-QacR contains an ATCC® synthetic promoter, pGPD- QacR, (ATCC SB-1071). They are two of DNA parts in the ATCC® Synthetic Biology Yeast Tool Kit for building gene circuits in yeast, Saccharomyces cerevisiae. Detail information of ATCC SB-1090 and SB-1071 can be found in these products’ information sheets.

Product Format purified plasmid DNA (2 to 3 µg/vial)
Storage Conditions 4°C or colder
Deposited as R. Weiss, Massachusetts Institute of Technology
References

Life Technologies™. Gateway® Technology User Manual. September 2003.

Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737

Ron Weiss, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Life Technologies™. Gateway® Technology User Manual. September 2003.

Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737

Ron Weiss, personal communication