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rtTA/TETO7 Activation Pair (ATCC® SB-2001)

Permits and Restrictions

View Permits

Components
Tet activator:
pENTR_L1L2_rtTA (ATCC SB-1031)

Inducible promoter:
pENTR_L4R1_pTETO7 (ATCC SB-1050)
Application A plasmid pair used for the construction of an inducible Tet-On gene expression system in the yeast Saccharomyces cerevisiae
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).

The Tet-On System is only responsive to doxycycline (Dox), not tetracycline.

Product Format purified plasmid DNA (2 to 3 µg/vial)
Storage Conditions 4°C or colder
Depositor R Weiss, Massachusetts Institute of Technology
References

Gossen M, et al. Transcriptional activation by tetracyclines in mammalian cells. Science 268(5218): 1766-1769, 1995. PubMed: 7792603

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gossen M, et al. Transcriptional activation by tetracyclines in mammalian cells. Science 268(5218): 1766-1769, 1995. PubMed: 7792603