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pENTR_L1L2_PhlF-VP64 (ATCC® SB-1099)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

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Designations pENTR_L1L2_PhlF-VP64
Depositors R Weiss, Massachusetts Institute of Technology
Applications A TetR homolog-based synthetic activator for gene expression regulation
Vector Total size of construct (kb): 3.414
Vector type: Entry vector
Markers: kanR
Insert Insert size (Kb): 0.81
Suggested primer for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
Preparation
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Related Products ATCC SB-1060
ATCC SB-1031
ATCC SB-1094
ATCC SB-1095
ATCC SB-1098
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
This construct contains a fusion of the TetR homolog PhlF and a VP64 activation domain.
References

Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737

Ron Weiss, personal communication