pENTR_L4R1_PhlF-pCYC1MIN (ATCC® SB-1060)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

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Designations pENTR_L4R1_PhlF-pCYC1MIN
Depositors R Weiss, Massachusetts Institute of Technology
Applications A TetR homolog-based synthetic promoter for gene expression regulation
Vector Total size of construct (kb): 2.955
Vector type: Entry vector
Markers: kanR
Insert Insert size (kb): 0.18
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
Related Products ATCC SB-1099
ATCC SB-1055
ATCC SB-1056
ATCC SB-1059
ATCC SB-1061
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).

This construct contains a synthetically designed inducible promoter comprising a TetR homolog PhlF operator and a yeast CYC1 minimal promoter. 

References

Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737

Ron Weiss, personal communication