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pENTR_L4R1_DONR2 (ATCC® SB-1014)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

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Designations pENTR_L4R1_DONR2
Depositors R Weiss, Massachusetts Institute of Technology
Applications

An empty entry vector of the Gateway® system for adding any promoter into the promoter library in the ATCC® Synthetic Biology Yeast Tool Kit. Gateway® is a registered trademark of Thermo Fisher Scientific.

Vector Total size of construct (kb): 3.361
Vector type: Donor vector
Markers: kanR
Insert Insert size (kb): 0.67
Suggested primer for sequencing:
5’- GTCCTACTCAGGAGAGC - 3’
Preparation
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Related Products ATCC SB-1013
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The vector contains a LacZ alpha gene flanked by two BsaI sites inserted between the two att sites (att L4 and att R1) for a Golden-Gate compatible cloning step to insert any promoter of interest.
References

Life Technologies™. Gateway® Technology User Manual. September 2003.