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pENTR_L4R1_pHOG1 (ATCC® SB-1008)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

View Permits

Designations pENTR_L4R1_pHOG1
Depositors R Weiss, Massachusetts Institute of Technology

An entry vector carrying a medium constitutive promoter for gene expression in Saccharomyces cerevisiae

Vector Total size of construct (kb): 3.448
Vector type: Entry vector
Markers: kanR
Insert Insert size (kb): 0.75
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
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ATCC SB-1104
ATCC SB-1105
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.


Ron Weiss, personal communication