To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org. For Technical questions please contact tech@atcc.org. Thank you.
X

pENTR_L1L2_KanMX (ATCC® SB-1006)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

View Permits

Designations pENTR_L1L2_KanMX
Depositors R Weiss, Massachusetts Institute of Technology
Applications An entry vector carrying a Kanamycin/G418-resistant gene for yeast transformation selection (detail information is described in the ATCC® Synthetic Biology Yeast Tool Kit Guide).
Vector Total size of construct (kb): 3.361
Vector type: Entry vector
Markers: kanR
Insert Insert size (kb): 0.81
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
Preparation
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Related Products ATCC SB-1100
ATCC SB-1031
ATCC SB-1032
ATCC SB-1085
ATCC SB-1086
ATCC SB-1089
ATCC SB-1090
ATCC SB-1094
ATCC SB-1095
ATCC SB-1098
ATCC SB-1099
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.