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pENTR_L1L2_KanMX (ATCC® SB-1006)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

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Designations pENTR_L1L2_KanMX
Depositors R Weiss, Massachusetts Institute of Technology
Applications An entry vector carrying a Kanamycin/G418-resistant gene for yeast transformation selection (detail information is described in the ATCC® Synthetic Biology Yeast Tool Kit Guide).
Vector Total size of construct (kb): 3.361
Vector type: Entry vector
Markers: kanR
Insert Insert size (kb): 0.81
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
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Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.