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L-M (ATCC® CCL-1.2)

Organism: Mus musculus, mouse  /  Tissue: subcutaneous connective tissue; areolar and adipose  /  Disease: normal

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue subcutaneous connective tissue; areolar and adipose
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 100 days
Gender male
Strain C3H/An
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Antigen Expression
H-2k
Virus Resistance
poliovirus 1, 2, 3
Comments
Grows in protein free medium.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Medium 199 with Earle's BSS supplemented with 0.5% Bacto-peptone
Subculturing
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Name of Depositor DJ Merchant
Deposited As Mus musculus
References

Kuchler RJ, Merchant DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures. Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956. PubMed: 13370534

Munyon WH, Merchant DJ. The relation between glucose utilization, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp. Cell Res. 17: 490-498, 1959. PubMed: 13672205

Merchant DJ, Hellman KB. Growth of L-M strain mouse cells in a chemically defined medium. Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962. PubMed: 14472756

Hsu TC, Merchant DJ. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J. Natl. Cancer Inst. 26: 1075-1083, 1961. PubMed: 13716385

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kuchler RJ, Merchant DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures. Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956. PubMed: 13370534

Munyon WH, Merchant DJ. The relation between glucose utilization, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp. Cell Res. 17: 490-498, 1959. PubMed: 13672205

Merchant DJ, Hellman KB. Growth of L-M strain mouse cells in a chemically defined medium. Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962. PubMed: 14472756

Hsu TC, Merchant DJ. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J. Natl. Cancer Inst. 26: 1075-1083, 1961. PubMed: 13716385

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.