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Escherichia coli GFP (ATCC® 25922GFP)

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Type Strain: no  /  Biosafety Level: 1

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Verified By Whole-genome Sequencing
Application Fluorescence detection
Fluorescence imaging
FACS
Cell infection assays
In vivo localization
Pathogen-host interactions assays
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serotype 06, Biotype 1
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antigenic Properties Serotype 06, Biotype 1
Antibiotic Resistance Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 100 µg/mL ampicillin.
Comments This clone was derived from ATCC® 25922™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling.

Vector: pUCP18-MCSgfpmut3
Vector size: 5.492kb
Vector type: plasmid
Marker: bla (ampR)
Features:
GFPmut3: fluorescent maker under the control of Plac promoter
Origin of replication: ori from pRO1600
Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI.
Double restriction sites: HindIII, PstI, SalI, XbaI.
Medium ATCC® Medium 2855: Tryptic Soy Agar/Broth with 100 mcg/ml Ampicillin
Growth Conditions Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor M Barbier
Chain of Custody Modified by ATCC (Dr. M. Barbier) <-- ATCC <-- FDA <-- F Schoenknecht
References

Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster.

Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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    Date Updated: 9/30/2019

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