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Enterovirus E (ATCC® VR-248)

Classification: Picornaviridae, Enterovirus  /  Product Format: frozen

Permits and Restrictions

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Classification Picornaviridae, Enterovirus
Deposited As Bovine enterovirus Type 1
Agent Enterovirus E
Strain LCR 4
Common Name Bovine enterovirus Type 1
Viruscide testing
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Cultivated readily in Am fluid of embryonated eggs. Produces paralytic disease in suckling mice closely resembling Coxsackievirus A disease.
Effect on Host
CPE, rounding and sloughing
Recommended Host
Monkey kidney cells; BT cells (ATCC® CRL-1390™)
Growth Conditions Temperature: 37°C 
Incubation: 1 to 3 days with 5% CO2 in air atmosphere recommended
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent)
Effect on Host
CPE, rounding and sloughing
Name of Depositor CM Kunin
Source Stool of healthy cow (Michigan)

Kunin CM, Minuse E. The isolation in tissue culture, chick embryo and suckling mice of filtrable agents from healthy dairy cattle. J. Immunol. 80: 1-11, 1958. PubMed: 13514066

Taylor MW, et al. Bovine enterovirus-1: characterization, replication and cytopathogenic effects. J. Gen. Virol. 23: 173-178, 1974. PubMed: 4364878

Chemical disinfectants and antiseptics --- Quantitative suspension test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary field --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 14675:2006.

Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution;British Standard BS EN 14885:2006.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation