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Enterococcus hirae Farrow and Collins (ATCC® 8043)

Strain Designations: R [BUCSAV 308, CCM 2423, CCM 2424, DSM 20160, JCM 8729, NCDO 1258, NCIB 6459, NCIB 8123]  /  Type Strain: yes  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Streptococcus lactis (Lister) Lohnis
Strain Designations R [BUCSAV 308, CCM 2423, CCM 2424, DSM 20160, JCM 8729, NCDO 1258, NCIB 6459, NCIB 8123]
Assay of folic acid
Assay of monensin
Quality control
Testing bactericides
Food testing
Water testing
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain yes
ATCC 9790 and ATCC 8043 are the same.
Medium ATCC® Medium 44: Brain Heart Infusion Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor EE Snell

AOAC International Monensin in feeds, turbidimetric method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 976.37, 1990

Farrow JA, Collins MD. Enterococcus hirae, a new species that includes amino acid assay strain NCDO 1258 and strains causing growth depression in young chickens. Int. J. Syst. Bacteriol. 35: 73-75, 1985.

AOAC International Vitamin assays, microbiological method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 960.46.

Water quality--Detection and enumeration of intestinal enterococci in surface and waste water--Part 1: Miniaturized method (MPN) by inoculation in liquid medium. Annex E. Quality criteria for manufacturing of the medium in microtitre plates. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 7899-1:1998.

Chemical disinfectants and antiseptics --- Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 1276:1997, 1997

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Cross References

Nucleotide (GenBank) : X87185 E.hirae 23S-5S rRNA spacer DNA.

Nucleotide (GenBank) : X87184 E.hirae 16S-23S rRNA spacer DNA.

Nucleotide (GenBank) : AX110254 Sequence 987 from Patent WO0123604.

Nucleotide (GenBank) : U39788 Enterococcus hirae D-alanine:D-alanine ligase gene, complete cds.

Nucleotide (GenBank) : AJ387916 Enterococcus hirae partial sodA gene for superoxide dismutase, strain CIP 53.48 T (ATCC 8043).

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation