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EWD299 (ATCC® 37218™)

Organism: Escherichia coli (Migula) Castellani and Chalmers  /  Clone Type: Clone  /  Depositors: WS Dallas

Permits and Restrictions

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Designations EWD299
Species Escherichia coli (Migula) Castellani and Chalmers
Depositors WS Dallas
Applications
detection of LT toxin
produces protein heat-labile enterotoxin
Vector
Construct size (kb): 10.10000038146973
Insert
DNA: genomic
Insert lengths(kb): 1.799999952316284
Gene product: heat-labile enterotoxin [lt]
Target Gene: LT toxin, heat-labile enterotoxin
Insert Size (kb) 1.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information

Shipped:  Freeze dried phagemid in E. coli

Comments
The insert contains internal HincII, EcoRI and HindIII sites.
The plasmid exists in DH1 as a multimer.
Digestion of the plasmid with HindIII yields a 0.85 kb fragment containing nucleotide sequence for the entire B subunit and about one-third of the A subunit.
This is a deletion mutant of EWD022 and seems to contain the minimum coding sequence to obtain functional proteins.
This expresses proteins of 22.5 kDa and 11.5 kDa, having both LT and adenylate cyclase-activating activity.
Classification Enterobacteriaceae, Escherichia
References

Dallas WS, et al. Cistrons encoding Escherichia coli heat-labile toxin. J. Bacteriol. 139: 850-858, 1979. PubMed: 383697

U.S. Food & Drug Administration Enterotoxigenic Escherichia coli: Heat-stabile enterotoxin (human), heat-stabile enterotoxin (porcine), and heat-labile enterotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed. Gaithersburg, MD. AOAC International pp. 24.12-24.14, 1995

AOAC International Detection of Escherichia coli producing heat-labile enterotoxin. DNA colony hybridization method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 984.34.

Guzman-Verduzco LM, Kupersztoch YM. Fusion of Escherichia coli heat-stable enterotoxin and heat-labile enterotoxin B subunit. J. Bacteriol. 169: 5201-5208, 1987. PubMed: 3312169