To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at For Technical questions please contact Thank you.

Privacy Policy Update

We remain dedicated to protecting your data and experience throughout our platforms. We have updated our Privacy Policy and your continued use of the Site means you have accepted the revised Privacy Policy. View now >

pBR313 (ATCC® 37018)

Applications: Food testing  /  Depositors: H Boyer

Designations pBR313
Permits and Restrictions

View Permits

Depositors H Boyer
Biosafety Level 1
Vector Information
Size (kb): 9.600
Vector: pBR313 (plasmid)
Construct size (kb): 9.600
Features: marker(s): ampR, tetR
replicon: pMB1
Food testing
General purpose plasmid vector.
Negative control for dectection of heat-labile enterotoxin.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Bolivar F, et al. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene 2: 75-93, 1977. PubMed: 344136

AOAC International Detection of Escherichia coli producing heat-labile enterotoxin. DNA colony hybridization method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 984.34.

Shipped frozen