Borrelia burgdorferi Johnson et al. emend. Baranton et al. (ATCC® 35210)

Strain Designations: B31  /  Type Strain: yes  /  Biosafety Level: 2

Permits and Restrictions

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Strain Designations B31
Emerging infectious disease research
Vector borne research
Tick, Ixodes dammini, New York
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genome Sequenced Strain

Borrelia burgdorferi B31, complete genome.

Type Strain yes
Genome sequencing strain
Absence of lipopolysaccharide
Lyme disease
In vitro cultivation
Survival in experimentally infected human blood
Genome sequenced strain
Infectivity of the strain in mice has not been confirmed at ATCC
Medium ATCC® Medium 1914: Revised BSK medium
Growth Conditions
Temperature: 37°C
Atmosphere: Microaerophilic
Name of Depositor AG Barbour
Chain of Custody
ATCC <-- AG Barbour <-- W Burgdorfer
Tick, Ixodes dammini, New York

Johnson RC, et al. Borrelia burgdorferi sp. nov.: Etiologic agent of Lyme disease. Int. J. Syst. Bacteriol. 34: 496-497, 1984.

Baranton G, et al. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int. J. Syst. Bacteriol. 42: 378-383, 1992. PubMed: 1380285

Barbour AG. Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med. 57: 521-525, 1984. PubMed: 6393604

Takayama K, et al. Absence of lipopolysaccharide in the Lyme disease spirochete, Borrelia burgdorferi. Infect. Immun. 55: 2311-2313, 1987. PubMed: 3623705

Johnson SE, et al. Borrelia burgdorferi: survival in experimentally infected human blood processed for transfusion. J. Infect. Dis. 162: 557-559, 1990. PubMed: 2373880

Burgdorfer W, et al. Lyme disease-a tick-borne spirochetosis. Science 216: 1317-1319, 1982. PubMed: 7043737

van Dam AP, et al. Complement-mediated serum sensitivity among spirochetes that cause Lyme disease. Infect. Immun. 65: 1228-1236, 1997. PubMed: 9119456

Shang ES, et al. Sequence analysis and characterization of a 40-kilodalton Borrelia hermsii glycerophosphodiester phosphodiesterase homolog. J. Bacteriol. 179: 2238-2246, 1997. PubMed: 9079909

Valsangiacomo C, et al. A phylogenetic analysis of Borrelia burgdorferi sensu lato based on sequence information from the hbb gene, coding for a histone-like protein. Int. J. Syst. Bacteriol. 47: 1-10, 1997. PubMed: 8995795

Barbour AG, et al. The nucleotide sequence of a linear plasmid of Borrelia burdorferi reveals similarities to those of circular plasmids of other prokaryotes. J. Bacteriol. 178: 6635-6639, 1996. PubMed: 8932323

Ferdows MS, et al. Conversion of a linear to a circular plasmid in the relapsing fever agent Borrelia hermsii. J. Bacteriol. 178: 793-800, 1996. PubMed: 8550515

Skare JT, et al. Porin activity of the native and recombinant outer membrane protein Oms28 of Borrelia burgdorferi. J. Bacteriol. 178: 4909-4918, 1996. PubMed: 8759855

Fraser CM, et al. Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390: 580-586, 1997. PubMed: 9403685

Bunikis J, et al. Surface exposure and species specificity of an immunoreactive domain of a 66-kilodalton outer membrane protein (P66) of the Borrelia spp. that cause lyme disease. Infect. Immun. 64: 5111-5116, 1996. PubMed: 8945554

Xu Y, et al. Correlation of plasmids with infectivity of Borrelia burgdorferi sensu stricto type strain B31. Infect. Immun. 64: 3870-3876, 1996. PubMed: 8751941

Roessner K, et al. Biased t-cell antigen receptor repertoire in lyme arthritis. Infect. Immun. 66: 1092-1099, 1998. PubMed: 9488400

Casjens S, et al. A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi. Mol. Microbiol. 32: 490-516, 2000. PubMed: 10672174

Western Blot Assay for Antibodies to Borrelia burgdorferi; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M34-A.

Bontemps-Gallo S, et al. Genomic and phenotypic characterization of Borrelia afzelii BO23 and Borrelia garinii CIP 103362. PLoS One 13(6):e0199641, 2018. PubMed: 29944685

Cross References

Nucleotide (GenBank) : M90084 Borrelia burgdorferi 22 kD antigen.

Nucleotide (GenBank) : M34710 Borrelia burgdorferei flagellin (fla) gene, 5' end.

Nucleotide (GenBank) : M59293 Borrelia burgdorferi small subunit ribosomal RNA sequence.

Nucleotide (GenBank) : U76405 Borrelia burgdorferi vmp-like vlsE (vlsE1) gene, complete cds.

Nucleotide (GenBank) : S75873 60 kda antigen [Borrelia coriaceae, Co53, ATCC 35210, Genomic, 1873 nt].

Nucleotide (GenBank) : U01894 Borrelia burgdorferi B31 outer surface protein C (ospC) gene, complete cds.

Nucleotide (GenBank) : U76406 Borrelia burgdorferi putative vls recombination cassettes Vls2-Vls16b (vls) gene, complete sequence.

Nucleotide (GenBank) : AE000783 Borrelia burgdorferi B31, complete genome.

Nucleotide (GenBank) : AE001115 Borrelia burgdorferi B31 (section 1 of 70) of the complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation