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Escherichia coli bacteriophage Phi X174 (ATCC® 13706-B1)

Strain Designations: Phi X174  /  Type Strain: no  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Phi X174
Strain Designations Phi X174
Host Organism
Escherichia coli strain C (ATCC 13706)
Control Culture
Resistance Testing
Coliphages in water
Control strain
Detection of bacteriophages
Reporter construct
Testing polyethylene film
Water testing
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Comments Somatic coliphage
Medium ATCC® Medium 129: Nutrient agar/stabs/broth with 0.5% NaCl
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor RL Sinsheimer

J. Mol. Biol. 1: 37-53, 1959.

Ullrich KA. Anti-viral acoustically transparent earphone cover. US Patent 5,545,859 dated Aug 13 1996

Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Two-Step Enrichment Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1601, 2001

Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1602, 2001

9224 B: Somatic Coliphages Assay. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA9224 - Detection of Coliphages;.

USEPA Manuual of Method for Virology, Chapeter 16. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (N16), 2001

Water quality--Detection and enumeration of bacteriophages--Part 2:Enumeration of somatic coliphages. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10705-2:2000.

Clothing for protection against contact with blood and body fluids--Determination of resistance of protective clothing materials to penetration by bloodborne pathogens--Test method using oX174 bacteriophage. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 16604:2004.

Standard Test Method for Resistance of Materials Used in Protetive Clothing to Penetration by Blood-borne Pathogens Using Phi-X174 Bateriophage Penetration as a Test System. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1671-07.

Water quality --- Detection and enumeration of bacteriophages --- Part 2: Enumeration of somatic coliphages [BS EN ISO 10705-2:2001]. London, UK:British Standards Institution;British Standard BS 6068-4.13:2000.

Fast Phage Test. Charm.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation