Credible solutions for critical public health emergencies
The outbreak of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) in December 2019 has put the health and safety of the global community at risk. The virus has already spread beyond the borders of China, with numerous countries reporting confirmed cases of coronavirus disease (COVID-19). With the virus continuing to spread and the number of confirmed cases and deaths rising, the World Health Organization has declared the outbreak a public health emergency of international concern.
As in previous public health emergencies such as Zika, SARS, MERS, and the H1N1 2009 pandemic, ATCC stands ready to partner with the dedicated scientists working toward preventing and containing this devastating outbreak. Only through the combined efforts of the global scientific community can we discover the tools and treatments needed to keep humankind healthy and safe. View our resources below to discover how we can support your work toward the development of novel diagnostics and effective therapeutics.
SARS-CoV-2 Genomic RNA and Heat-Inactivated Strain
Clinically relevant reference materials are an essential component of basic research and diagnostic development. That’s why ATCC has made it a priority to provide heat-inactivated and genomic RNA preparations from a strain recently sourced from an infected patient in Washington state (2019-nCoV/USA-WA1/2020). This strain is fully sequenced (GenBank: MN985325.1) and serves as the SARS-CoV-2 reference material for the United States.
Synthetic Molecular Standards for SARS-CoV-2
Effective immediately, VR-3276SD™ will be replacing VR-3276T™ in our catalog.
- VR-3276SD™ is identical to VR-3276T™ in construction and sequence.
- VR-3276SD™ differs from VR-3276T™ only in the operational designation. Whereas VR-3267T™ is an ISO 9001 manufactured product, VR-3276SD™ is manufactured under ISO 13485 guidelines and is therefore suitable for a broader range of usage.
Because the transmission dynamics and pathogenic nature of SARS-CoV-2 are not yet fully known, the utmost care should be taken when handling the virus. Current guidelines from the CDC recommend that the virus should only be handled in a biosafety level 3 facility. While these strict containment requirements ensure the safety of laboratory workers, it has made developing novel molecular-based detection methods challenging.
To address this, ATCC developed three quantitative synthetic molecular standards for use as controls in the development of molecular assays designed to detect and quantify SARS-CoV-2. Because these standards are synthetically derived, they eliminate the need to culture viruses in the laboratory and they are safe to use under biosafety level 1 conditions.
These constructs were developed under ISO 13485 guidance and comprise fragments representing different regions of the SARS-CoV-2 genome. Further, SARS-CoV-2 construct 1 is compatible with the nucleocapsid-targeted real-time RT-PCR primer and probe sets developed by the CDC.
Coronavirus Research Materials
When developing a new assay to detect SARS-CoV-2, having access to other coronavirus strains can be valuable for establishing inclusivity and exclusivity. To support this need, ATCC provides viral strains and nucleic acids representing alphacoronaviruses and betacoronaviruses known to cause disease in humans.
We also offer a variety of coronavirus strains known to cause disease in feline, avian, and murine hosts, as well as the cell lines and media needed to cultivate them. These materials provide researchers with the tools needed to enhance the development of their assays.
Cell Lines for SARS-CoV-2 Propagation
To develop new vaccines or test antiviral compounds, researchers need access to virus isolates. However, during the outbreak of a novel virus like SARS-CoV-2, it can be challenging to determine which propagation host is ideal for successful viral replication. In a recent study by Harcourt J, et al., it was discovered that SARS-CoV-2 can replicate to a high titer in Vero CCL-81 and Vero E6 cells in the absence of trypsin. These cell lines and the media needed to cultivate them are available at ATCC—explore our collection today.
Cell Lines for Enhanced Virus Production
The continual spread of deadly viruses necessitates the development of novel prevention and treatment options. However, the development of a new antiviral vaccine can be challenged by low-yielding manufacturing processes. To address this, ATCC used cutting-edge CRISPR/Cas9 gene-editing technology to develop STAT1 knockout cell lines capable of producing high-titer viral stocks. Discover how these advanced biological models can be used in your vaccine development research.
Your project is unique. That’s why ATCC offers customized services and solutions to meet your specific assay development needs. We can help you develop, characterize, manufacture, and store the microorganisms, cell lines, and nucleic acids that support your essential research. From biobanking and authentication to developing controls and custom nucleic acids—we have the solutions you need to support every stage of your project.
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