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Genomic RNA from Severe acute respiratory syndrome-related coronavirus 2 (ATCC® VR-3326D)

Product Format: frozen
100 µL per vial

Agent Genomic RNA from Severe acute respiratory syndrome-related coronavirus 2
Strain USA/CA_CDC_5574/2020
Verified By Whole-genome Sequencing
Applications ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
100 µL per vial
Storage Conditions -70°C or colder
Genome Sequenced Strain

Yes

Comments

Genomic RNA isolated from a preparation of Severe acute respiratory syndrome-related coronavirus 2 isolate USA/CA_CDC_5574/2020.

This isolate is lineage B.1.1.7, clade GR according to GISAID (Global Initiative on Sharing All Influenza Data) conventions.

The following mutations were identified in the clinical isolate: Spike A570D, Spike D614G, Spike D1118H, Spike H69del, Spike N501Y, Spike P681H, Spike S982A, Spike T716I, Spike V70del, Spike Y145del, M (Membrane protein) V70L, N (Nucleocapsid protein) D3L, N G204R, N R203K, N S235F, NS3 T223I, NS8 (Non-structural protein 8) Q27stop, NS8 R52I, NS8 Y73C, NSP3 (Non-structural protein 3) A890D, NSP3 I1412T, NSP3 T183I, NSP6 (Non-structural protein 6) F108del, NSP6 G107del, NSP6 S106del, NSP12 (Non-structural protein 12) P323L, NSP13 (Non-structural protein 13) A454V, NSP13 K460R.

One additional SNP in ORF1ab L3826F was reported in the deposited passage two virus, in comparison to the clinical specimen.

Genomic RNA from SARS-CoV-2 must be handled at BSL-2 containment.

Innocuity testing was performed on the extracted RNA and results reported on the Certificate of Analysis.

Name of Depositor CDC
References

Rambaut A, et al. A Dynamic Nomenclature Proposal for SARS-CoV-2 Lineages to Assist Genomic Epidemiology. Nat Microbiol 5: 1403-1407, 2020. PubMed: 32669681

Mercatelli D and Giorgi FM. Geographic and Genomic Distribution of SARS-CoV-2 Mutations. Front Microbiol: doi.org/10.3389/fmicb.2020.01800, 2020. PubMed: 32793182

Gralinski LE and Menachery VD. Return of the Coronavirus: 2019-nCoV. Viruses 12: 135, 2020. PubMed: 31991541