Bourbon virus (ATCC® VR-1842)

Classification: Orthomyxoviridae, Thogotovirus  /  Product Format: frozen 1.0 mL per vial

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Classification Orthomyxoviridae, Thogotovirus
Agent Bourbon virus
Strain Original
Biosafety Level 3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C
Comments BRBV is a newly discovered, potentially tick-borne human pathogen associated with febrile illness and death in the index case
Effect on Host
CPE, rounding and sloughing
Recommended Host
Vero C1008 cells [Vero 76, clone E6, Vero E6] (ATCC® CRL-1586™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells approximately 24 hours prior to infection and infect when cultures are 90-100% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 3 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 90% of the monolayer.

Effect on Host
CPE, rounding and sloughing
Name of Depositor BJ Russell, Centers for Disease Control and Prevention
Source Isolated from a human male with fever, thrombocytopenia, and a recent history of tick exposure in Bourbon County, Kansas, in June 2014.
References

Lambert AJ, et al. Molecular, serological and in vitro culture-based characterization of Bourbon virus, a newly described human pathogen of the genus Thogotovirus. J Clin Virol 73: 127-132, 2015. PubMed: 26609638

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