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Legionella pneumophila subsp. pneumophila Brenner et al. (ATCC® 33152)

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Strain Designations: Philadelphia-1  /  Type Strain: yes  /  Biosafety Level: 2

Permits and Restrictions

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Verified By Whole-genome Sequencing
Strain Designations Philadelphia-1
Media testing
Emerging infectious disease research
Respiratory research
Human lung
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Genome Sequenced Strain

Legionella pneumophila subsp. pneumophila str. Philadelphia 1, complete genome.

Type Strain yes
Genome sequenced strain
Fluorescent antibody serogroup 1
Fatty acid composition
Medium ATCC® Medium 1099: CYE (Charcoal Yeast Extract) Buffered Medium
Growth Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Name of Depositor RE Weaver
Chain of Custody
ATCC <-- RE Weaver <-- JE McDade
Human lung

Validation list no. 3. Int. J. Syst. Bacteriol. 29: 436, 1979.

Validation list no. 29. Int. J. Syst. Bacteriol. 39: 205-206, 1989.

Brenner DJ, et al. Classification of the Legionnaires' disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann. Intern. Med. 90: 656-658, 1979. PubMed: 434652

Moss CW, et al. Cellular fatty acid composition of isolates from Legionnaires disease. J. Clin. Microbiol. 6: 140-143, 1977. PubMed: 893658

Brenner DJ, et al. Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov.. J. Clin. Microbiol. 26: 1695-1703, 1988. PubMed: 3053773

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Cross References

Nucleotide (GenBank) : X72354 L.pneumophila Sgp.1 (ATCC 33152) gene for 5S ribosomal RNA.

Nucleotide (GenBank) : U68079 Legionella pneumophila coded portion of proteolysis tag tmRNA gene.

Nucleotide (GenBank) : AX109589 Sequence 322 from Patent WO0123604.

Nucleotide (GenBank) : AX111039 Sequence 1772 from Patent WO0123604.

Nucleotide (GenBank) : M59157 Legionella pneumophila 16S ribosomal RNA.

Nucleotide (GenBank) : Z24705 L.pneumophila 23S and 5S rRNA genes, partial CDS's.

Nucleotide (GenBank) : AJ011775 Legionella pneumophila djlA and kdtA (waaA) genes.

Nucleotide (GenBank) : M19444 L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 2 of 3.

Nucleotide (GenBank) : M19450 L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 1 of 3.

Nucleotide (GenBank) : M22266 L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 3 of 3.

Nucleotide (GenBank) : Z30431 L.pneumophila Serogroup 1 Philadelphia-1 gene for 23S ribosomal RNA and 5S ribosomal RNA.

Nucleotide (GenBank) : AF095231 Legionella pneumophila strain ATCC 33152 defect in organelle trafficking protein (dotA) gene, partial cds.

Nucleotide (GenBank) : AE017354 Legionella pneumophila subsp. pneumophila str. Philadelphia 1, complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019