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1.800.638.6597

U-87 MG (ATCC^® HTB-14^™)

Organism: Homo sapiens, human / Tissue: brain / Disease: Likely glioblastoma

U-87 MG ATCC^® HTB-14^™

frozen

For-Profit: $495.00 Non-Profit: $420.75

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Permits and Restrictions	View Permits
Organism	Homo sapiens, human
Tissue	brain
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Likely glioblastoma
Age	unknown
Gender	male
Ethnicity	unknown
Applications	This cell line is a suitable transfection host.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found.
Images
Derivation	This is one of a number of cell lines derived from malignant gliomas (see also ATCC^® HTB-15™ and HTB-16™) by J. Ponten and associates from 1966 to 1969 (see also Allen, 2016).
Clinical Data	male
Tumorigenic	Yes
Effects	Yes, in nude mice inoculated subcutaneously with 10(7) cells
Comments	Mycoplasma contamination was eliminated in September 1975. The ATCC^® HTB-14™ cell line was deposited at ATCC in 1982. STR profiling, Y-chromosome paint, and Q-band assay confirmed that the cell line is male in origin. Based on current literature, the cell line is likely a glioblastoma of CNS origin (Allen, 2016).

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes used in this protocol are for 75 cm²flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Culture medium, 95%; DMSO, 5%
Culture Conditions	Atmosphere: 5% CO₂ in air recommended Temperature: 37°C

STR Profile	Amelogenin: X,Y CSF1PO: 10,11 D13S317: 8,11 D7S820: 8,9 D5S818: 11,12 D16S539: 12 vWA: 15,17 TH01: 9.3 TPOX: 8
Isoenzymes	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 2 PGM3, 1

Name of Depositor	J Ponten
Deposited As	Homo sapiens
Year of Deposit	1966
References	Allen M, et al. Origin of the U87MG glioma cell line: Good news and bad news. Sci. Trans. Med. 8(354): 1-4, 2016. Clark MJ, et al. U87MG decoded: The genomic sequence of a cytogenetically aberrant human cancer cell line. PLoS Genetics 6 (1) : e1000832, 2010. Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306 Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744 Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504 Tumors developed within 21 days at 100% frequency (5/5).
Cross References	Nucleotide (GenBank) : E02404 DNA encoding exon K of NAP. Nucleotide (GenBank) : X06981 Human mRNA fragment for amyloid beta-protein (AP) insertion. Nucleotide (GenBank) : E02405 DNA encoding PI(the active region that has protease inhibitor activity of NAP). Nucleotide (GenBank) : E02400 DNA encoding NAP(new senile plaque amyloid precursor protein having protease inhibitor). Nucleotide (GenBank) : E02402 DNA encoding exon I of NAp(new senile plaque amyloid precursor having protease inhibitor). Nucleotide (GenBank) : E02401 DNA encoding exon H of NAp(new senile plaque amyloid precursor protein having protease inhibitor). Nucleotide (GenBank) : E02403 DNA encoding exon J of NAP(new senile plaque amyloid precursor protein having protease inhibitor).

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
Other Documentation	Cell Micrograph Cancer cell line mutation data
FAQ's	ATCC HTB-14 morphology and growth conditions Date Updated: 12/18/2017
References	Allen M, et al. Origin of the U87MG glioma cell line: Good news and bad news. Sci. Trans. Med. 8(354): 1-4, 2016. Clark MJ, et al. U87MG decoded: The genomic sequence of a cytogenetically aberrant human cancer cell line. PLoS Genetics 6 (1) : e1000832, 2010. Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306 Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744 Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504 Tumors developed within 21 days at 100% frequency (5/5).