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iPSC-derived Monocytes; DYS0100 (ATCC® ACS-7030)

Organism: Homo sapiens, human  /  Tissue: iPSCs derived from foreskin fibroblasts  /  Disease: normal

Permits and Restrictions

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Organism Homo sapiens, human
Tissue iPSCs derived from foreskin fibroblasts
Product Format frozen 1.0 mL
Morphology rounded
Culture Properties suspension
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age newborn
Gender male
Ethnicity unknown
Applications Biopharma: Cancer immunology, drug development, and toxicity screening
Storage Conditions liquid nitrogen vapor phase
Comments

Human iPSC-derived Monocytes enables researchers to carry out application which are done using primary CD14+ monocytes. These cells can be used for differentiating into functionally active macrophages and dendritic cells. Additionally these cells can be used for MAT assay, drug development, toxicity screening, and cancer immunology experiments. There is an availability of large number of cell vials from a single donor.

Complete Growth Medium iPSC-derived Monocytes have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining iPSC-derived Monocytes cells in culture in the absence of application-specific growth factors.
Cryopreservation N/A: As this cell line is intended to be consumable no sub culturing and no cryopreservation is recommended.
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial Approximately 2.5 x 106 cells
Volume 1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 7,11
D13S17: 11,13
D16S539: 12
D5S818: 11
D7S820: 9,10
TPOX: 8,11
TH01: 9.3
vWA: 14,19
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viral Testing Hepatitis B: None detected
Hepatitis C: None detected
Human immunodeficiency virus 1: None detected
Human immunodeficiency virus 2: None detected
Viability ≥ 80%
Functional Tests Phagocytosis assay; macrophage and dendritic cell differentiation
Name of Depositor ATCC
Year of Origin 2018
References

Panicker LM, et al. Gaucher iPSC derived macrophages produce elevated levels of inflammatory mediators and serve as a new platform for therapeutic development. Stem Cells 32(9): 2338-49, 2014. PubMed: 24801745

Spiller KL, et al. Differential gene expression in human, murine and cell line-derived macrophages upon polarization. Exp Cell Res 347(1): 1-13, 2015. PubMed: 26500109

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

This product may be used by investigator for research purposes subject to the Limited Use Label License and any additional third party terms. This product is subject to claims under U.S. Patent Nos. 8,058,065 and 8,048,999, pending patent applications, and foreign counterparts thereof.  In addition, this product was generated using the proprietary technology of the DNAVEC Corporation, including, without limitation, recombinant Sendai virus vectors. For information on obtaining additional rights, please contact licensing@atcc.org


References

Panicker LM, et al. Gaucher iPSC derived macrophages produce elevated levels of inflammatory mediators and serve as a new platform for therapeutic development. Stem Cells 32(9): 2338-49, 2014. PubMed: 24801745

Spiller KL, et al. Differential gene expression in human, murine and cell line-derived macrophages upon polarization. Exp Cell Res 347(1): 1-13, 2015. PubMed: 26500109