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hTERT Immortalized RPTEC Growth Kit (ATCC® ACS-4007)

Permits and Restrictions

View Permits

Product Format frozen
Storage Conditions -20°C
Shipping Information Frozen supplement kit for 500 mL base medium
Applications Add to DMEM:F12 (ATCC® 30-2006™) to produce complete medium for hTERT Immortalized RPTEC cells (ATCC® CRL-4031™)

Comments Each growth kit contains:
PCS-999-058 5 ml RPTEC Supplement A
PCS-999-059 10 ml RPTEC Supplement B

Required but not supplied: G418 solution MUST be added to the above medium to a final concentration of 0.1 mg/mL G418 to maintain the selective pressure for immortalization 

The hTERT immortalized RPTEC Growth Kit contains components that when added to DMEM:F12 (ATCC® 30-2006™) create a complete cell culture environment for hTERT immortalized RPTEC cells (ATCC® CRL-4031™). The final concentration for each growth kit component in the complete hTERT immortalized RPTEC growth medium is as follows:
25 ng/mL Hydrocortisone
3.5 µg/mL Ascorbic Acid
8.65 ng/mL Sodium selenite
5.0 µg/mL Transferrin
5.0 µg/mL insulin
5 pM Triiodo-L-thyronine
25 ng/mL Prostaglandin E1
10 ng/mL rhEGF
1.2 mg/mL Sodium Bicarbonate 

Note: Fetal Bovine Serum is not required for proliferation but may be added if it is desired to transfect the cells (final FBS concentration in complete medium is 0.2%).

Biosafety Level N/A
Sterility Testing Negative for bacteria, fungi, and yeast
Functional Testing Rate of proliferation and morphology comparable to control. Cells cultured in complete medium exhibit dome structures in 7-14 days
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Wieser M, et al. hTERT alone immortalizes epithelial cells of renal proximal tubules without changing their functional characteristics. Am. J. Physiol. Renal Physiol. 295: 1365-1375, 2008. PubMed: 18715936