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Neural Progenitor Cell Origin ATCC-BXS0117 Normal; Human (ATCC® ACS-5003)

Organism: Homo sapiens, human  /  Cell Type: neural progenitor cells  /  Tissue: bone marrow CD34+ cells  /  Disease: normal

Permits and Restrictions

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Organism Homo sapiens, human
Tissue bone marrow CD34+ cells
Cell Type neural progenitor cells
Product Format frozen 1.0 mL per vial
Morphology short spindle shape
Culture Properties adherent
Biosafety Level 2  [Cells contain Sendai viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 27
Gender female
Ethnicity Chinese
Applications Neuronal differentiation and drug screen
Storage Conditions Liquid nitrogen vapor phase (-130°C or colder)
Images ACS-5003 Micrograph ACS-5003 Astrocyte Markers ACS-5003 Dopaminergic Markers ACS-5003 NPC Markers ACS-5003 Oligodendrocyte Markers
Derivation Normal NPCs (ATCC® ACS-5003™) are derived from ATCC-BXS0117 Human [Asian Female] Induced Pluripotent Stem Cells (ATCC® ACS-1031™)
Antigen Expression Nestin, Pax-6
Comments Astrocyte, oligodendrocyte, and neuron differentiation; drug screening
Complete Growth Medium

Complete growth media for Neural Progenitor Cells (NPCs) includes DMEM: F12 (ATCC® 30-2006) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003). To make complete NPC medium add the following components of the kit to 464 mL DMEM: F12:

5 mL L-Alanyl-L-Glutamine
5 mL Non-Essential Amino Acids
10 mL NPC Growth Kit Component A
5 mL NPC Growth Kit Component B
1 mL NPC Growth Kit Component C
10 mL NPC Growth Kit Component D
Seeding Density Post-thaw: 80,000 viable cells/cm2 on CellMatrix-coated dishes/plates.
Subculture: 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates.
Subculturing

Post thaw day 1, perform a 100% medium change and remove all cells that did not attach. Perform a 100% medium change every other day thereafter. Passage the cells cells with diluted Accutase (50% Accutase and 50% DPBS) when they reach ~95% confluence and reseed the NPCs at 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates.

Cryopreservation Stem Cell Freezing Media (ATCC® ACS-3020™)
Cells per Vial >1,000,000 cells per vial
Volume 1.0 mL per vial
STR Profile Amelogenin: X
CSF1PO: 10,12
D13S17: 9,12
D16S539: 11
D5S818: 12
D7S820: 9,13
TH01: 7,9
TPOX: 8,11
vWA: 19
Sterility Tests

No bacterial or fungal growth after 21 days

Viral Testing None detected
Viability ≥80% viability
Functional Tests Exhibit differentiation potential of NPCs into Tuj1+ early neurons and TH+ dopaminergic neurons
Passage Number P4
Population Doubling Capacity ≥15 PDLs
Population Doubling Time ~38 hours
Name of Depositor ATCC
Year of Origin 2015
References

Malik N, et al. Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons. Neurotoxicology 45: 192-200, 2014. PubMed: 25454721

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

This product may be used by investigator for research purposes subject to the Limited Use Label License and any additional third party terms. This product is subject to claims under U.S. Patent Nos. 8,058,065 and 8,048,999, pending patent applications, and foreign counterparts thereof.  In addition, this product was generated using the proprietary technology of the DNAVEC Corporation, including, without limitation, recombinant Sendai virus vectors. For information on obtaining additional rights, please contact licensing@atcc.org

References

Malik N, et al. Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons. Neurotoxicology 45: 192-200, 2014. PubMed: 25454721