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Neuronal Progenitor Cells Derived from XCL-1 MAP2p-Nanoluc-Halotag (ATCC® ACS-5007)

Organism: Homo sapiens, human  /  Cell Type: neural progenitor cells  /  Tissue: CD34+ cord blood  /  Disease: normal

Permits and Restrictions

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Organism Homo sapiens, human
Tissue CD34+ cord blood
Cell Type neural progenitor cells
Product Format frozen 1.0 mL per vial
Morphology short spindle shape
Culture Properties adherent
Biosafety Level 2  [Cells contain Sendai viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age newborn
Gender male
Ethnicity Caucasian
Applications Neuronal differentiation and drug screening; Quantitative measurement of neuronal differentiation with Nano Luciferase expression under the control of the MAP2 gene promoter.
Intended Use Astrocyte, oligodendrocyte, and neuron differentiation; drug screening; Quantitative measurement of ealy neuron differentiation.
Storage Conditions Liquid nitrogen vapor phase (-130°C or colder)
Images ACS-5007 Micrograph ACS-5007 Differentiation Markers ACS-5007 Halotag ACS-5007 MAP2 Halotag ACS-5007 Nanoluc ACS-5007 NPC Markers
Derivation

ACS-5007 NPCs are derived from NIH NCRM-1 iPSC line

Antigen Expression Nestin, Pax-6
Complete Growth Medium

Complete growth media for Neural Progenitor Cells (NPCs) includes DMEM: F12 (ATCC® 30-2006) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003). To make complete NPC medium add the following components of the kit to 464 mL DMEM: F12:

5 mL L-Alanyl-L-Glutamine
5 mL Non-Essential Amino Acids
10 mL NPC Growth Kit Component A
5 mL NPC Growth Kit Component B
1 mL NPC Growth Kit Component C
10 mL NPC Growth Kit Component D
Seeding Density Post-thaw: 80,000 viable cells/cm2 on CellMatrix-coated dishes/plates.
Subculture: 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates.
Subculturing

Post thaw day 1, perform a 100% medium change and remove all cells that did not attach. Perform a 100% medium change every other day thereafter. Passage the cells cells with diluted Accutase (50% Accutase and 50% DPBS) when they reach ~95% confluence and reseed the NPCs at 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates.

Cryopreservation

Post thaw day 1, perform a 100% medium change and remove all cells that did not attach. Perform a 100% medium change every other day thereafter. Passage the cells cells with diluted Accutase (50% Accutase and 50% DPBS) when they reach ~95% confluence and reseed the NPCs at 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates.

Cells per Vial >1,000,000 cells per vial
Volume 1.0 mL per vial
STR Profile Amelogenin: X,Y
CSF1PO: 9,10
D13S17: 9,10
D16S539: 9,13
D5S818: 14,16
D7S820: 8,9
TH01: 9.3
TPOX: 8,11
Sterility Tests

No bacterial or fungal growth after 21 days

Viral Testing

None detected

Viability ≥80% viability
Functional Tests Exhibit differentiation potential of NPCs into Tuj1+ early neurons and TH+ dopaminergic neurons
Population Doubling Capacity ≥15 PDLs
Name of Depositor ATCC
Year of Origin 2015
References

Malik N, et al. Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons. Neurotoxicology 45: 192-200, 2014. PubMed: 25454721

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

This product may be used by investigator for research purposes subject to the Limited Use Label License and any additional third party terms. This product is subject to claims under U.S. Patent Nos. 8,058,065 and 8,048,999, pending patent applications, and foreign counterparts thereof.

This material is subject to the following restrictions in addition to those outlined in the ATCC Material Transfer Agreement:

  1. HaloTag Label License

For information on obtaining additional rights, please contact:

ATCC Licensing
Email: licensing@atcc.org

References

Malik N, et al. Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons. Neurotoxicology 45: 192-200, 2014. PubMed: 25454721