Organoid Culture Fundamentals: Critical Steps for Success

3/25/2021


March 25, 12:00 PM EST

Abstract

The currently available preclinical cancer models are often inadequate for studying cancer biology, developing personalized therapeutics, and identifying unique biomarkers. To address this deficit, ATCC has collaborated with the Human Cancer Model Initiative (HCMI) to launch almost 150 patient-derived models, featuring over 80 organoids. The clinical and sequencing data for these advanced models are available through a linked portal to the HCMI searchable catalog and the Genomic Data Commons (GDC) data portal. In addition to providing the models, ATCC supports scientists who are new to culturing these complex microtissues by offering organoid culture protocols and complete medium formulations. In this webinar we present step-by-step instructions that thoroughly demonstrate the process of thawing, expanding, and cryopreserving organoids.

Key points:

  • Clinical and sequencing data for the HCMI models are available through a linked portal to the HCMI searchable catalog and the GDC data portal.
  • While 3D organoid culture may seem intimidating, careful preparation and good work instructions, such as those contained in this webinar, will start you on the path to successfully culturing this advanced type of in vitro model. 
  • ATCC further supports the HCMI models by offering organoid "CoreKits" that contain the necessary reagents for organoid expansion.

Presenter

Steve Budd

Steven Budd, M.S., M.B.A.,
Product Line Business Specialist, ATCC

Steven Budd is a Product Line Business Specialist that manages the cell culture reagents at ATCC. He has 4 years of experience in the product management of scientific tools. Before that, he gained 4 years of experience in biomedical research and cell culture as a research specialist at the University of North Carolina at Chapel Hill. Mr. Budd has a M.S. in Biology from the University of North Carolina at Wilmington and an M.B.A. from North Carolina State University.

James Clinton, Ph.D.

James Clinton, Ph.D.,
Senior Scientist, ATCC

James Clinton, Ph.D., works in new product development, with a focus on primary cells and advanced, physiologically relevant culture systems using novel technologies. Previously he worked at University of California, San Diego and the La Jolla Institute for Molecular Medicine. Dr. Clinton attended Washington State University and University of California, San Diego where he studied Neuroscience.