Biology of Anaerobic Bacteria and Predominant Propagation Practices


Abstract: Culturing anaerobes in the laboratory can be challenging. Due to the copious growth requirements and the plethora of anaerobic organisms, knowing the optimal conditions for your anaerobic organism is essential. In this webinar, we will present the various methods to achieve successful growth conditions for a wide variety of anaerobes. We will consider topics such as common anaerobic gas mixtures, media selection, and obtaining anaerobic conditions in the lab. Taking into account individual laboratory constraints, we will also discuss several propagation methods available today. Using specific examples from the ATCC collection, we will demonstrate these techniques.

Key Points:

  • There are numerous types of anaerobic bacteria with various atmospheric and nutritional requirements from clinical gut and wound isolates to environmental extremophiles.
  • Creating and maintaining anaerobic atmospheric and nutritional conditions are critical for the successful growth of anaerobic bacteria.
  • The propagation of anaerobic bacteria has evolved throughout the years. The discovery of new isolates has brought about the need for more robust culturing technology.


Allison Faust

Allison Faust,
Senior Biologist, ATCC

Allison Faust is a Senior Biologist in the Laboratory Testing Services laboratory at ATCC. She has a B.S. in Biology and extensive experience in culturing and quality control testing the vast array of bacterial and protist isolates offered at ATCC. She has participated in anaerobic workshops and internal anaerobic studies to optimize the anaerobes offered at ATCC.

Nancy Krueger

Nancy Krueger,
Senior Biologist, ATCC

Nancy Krueger is a Senior Biologist in the Manufacturing, Science, and Technology (MSAT) Bacteriology department at ATCC. During the last 11 years, she has developed an in-depth knowledge of technology transfer, quality control, and propagation of a variety of bacteria, fungi, and yeast. Ms. Krueger is also experienced in studies determining the ideal propagation and test methods for anaerobes, optimization of the propagation methods of bacteriophage, and enumeration of Mycoplasma. Prior to working at ATCC, she worked in healthcare in a hospital laboratory and emergency department. Ms. Krueger has a bachelor’s degree in Biology from George Mason University.