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Abstract:

Kidney membrane transporters are key to drug disposition and renal clearance. Primary renal proximal tubule epithelial cells (RPTEC) are the most physiologically relevant cell models, but lose OAT1 and OCT2 expression in culture. Primary RPTEC transiently expressing these transporters show large variations between production lots. Furthermore, cell line-based models either do not have the kidney tissue origination or are tumor-derived. This presentation will introduce transporter cell models using hTERT-immortalized RPTEC that stably overexpress the OAT1 or OCT2 gene. Our data show that these cell lines provide tissue-relevant results, improved consistency over time, and predictability for clinical trials.

Key Points:

  • There is a lack of in vitro models that durably and correctly recapitulate kidney physiology
  • ATCC has created kidney cell models using hTERT-immortalized RPTEC that stably overexpress the OAT1 or OCT2 gene
  • hTERT-immortalized RPTEC provide kidney tissue-related results, improved consistency over time, and predictability for clinical trials

10/12/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Employing good aseptic technique and understanding the specific culture requirements of a strain are essential for optimal viral growth. In this webinar, we will discuss the basic principles for propagating animal viruses in the laboratory. Here, we will highlight best practices for making good virus stocks with an emphasis on the art and science of virus culture that should be followed to help avoid contamination and cross-contamination. Further, we will provide an in-depth look at viral culturing practices in both cell culture and embryonated chicken eggs using Influenza virus as an example.

Key Points:

  • Good aseptic technique alone is not sufficient to grow and maintain virus stocks
  • Host cell culture authentication is important for growing a good stock of virus
  • The nature of biomaterials used in culturing viruses in cells and the tendency for viruses to mutate are the primary challenges in obtaining consistent end results

Time: 12:00 PM Eastern Standard Time

Abstract:

Over the past few decades, much has been written about misidentification of cell lines, primarily human cell lines. It is not unusual that studies of the ‘same’ cell lines performed by different laboratories often show different results using the same methodology. Thus far, numerous cell lines are known to be misidentified due, in part, to lack of adequate testing. The financial loss incurred by using misidentified cell lines is estimated in the millions of dollars. Cell line authentication is crucial. The validity of scientific data demands that consistent and unequivocal verification of cell line identity is precise. The implementation of best tissue culture practices to include routine authentication of human cell lines by short tandem repeat (STR) profiling is highly recommended by funding agencies, journal editors, and scientific societies. This presentation will focus on the use of STR Profiling for human cell line authentication.

Key Points:

  • Misidentification of cell lines may result in invalidated research results, withdrawal of funding, publication retraction, and loss of reputation
  • Authentication of cell lines is critical to trusting your data
  • STR profiling is an inexpensive, reliable method to authenticate human cell lines

Time: 12:00 PM Eastern Standard Time

Abstract:

The recent development of the CRISPR/Cas9 system provides a revolutionary gene-editing technology for basic research in biology and for development of targeted cancer therapies. In addition to enabling the identification of novel drug targets through functional screening, CRISPR/Cas9 facilitates the creation of disease models for drug discovery and development.

In this webinar, ATCC experts will address how ATCC utilized this advanced technology to create novel human cell models that contain disease-relevant point mutations and gene rearrangements. In addition, we will introduce a new type of BRAF inhibitor-resistant cell line that was created by using CRISPR/Cas9 to insert the NRAS Q61K mutation. These human isogenic lines provide useful disease models for the identification and validation of new therapeutics.

Key Points:

  • CRISPR/Cas9 gene editing technology is a powerful tool for drug discovery
  • Gene editing technology can be used to create disease-relevant cell models for screening new anti-cancer drug targets
  • CRISPR/Cas9 is a useful tool for creating new types of drug-resistant cell models

Fungal Systematics

Current Technologies and Techniques for Fungal Identification

9/21/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Recent updates in the nomenclature rules for fungi (Melbourne Code, 2012) and improvements in contemporary molecular techniques have significantly affected fungal systematics and has triggered numerous taxonomic revisions among yeasts and fungi species in recent years. To deliver accurate and up-to-date information to our customers, we diligently characterize ATCC mycology products not only through rigorous quality control procedures, but also by various research projects. In this webinar, we will discuss how to identify fungi effectively and accurately under the current nomenclatural system by introducing a few recent studies on medically and industrially relevant yeasts and fungi.

Key Points:

  • The new ‘One fungus, One name’ rule has significantly affected fungal systematics
  • The quality control procedures used to evaluate and characterize ATCC mycology products meet the high standard of industrial demands
  • Recent studies support MALDI-TOF mass spectrometry as a powerful rapid identification tool for yeasts and fungi

Time: 12:00 PM Eastern Standard Time

Abstract:

Assessing cell viability and cell health is crucial to all aspects of cell culture. In addition to the proper maintenance of cell stocks, cell health practices increase the level of confidence in experimental results. In this webinar presentation, we will discuss reliable and economic methods for determining cell proliferation and cell viability. Other cell culture practices and issues that affect cell health will also be addressed. These topics include choices in cell culture media/reagents, cryopreservation factors that affect cell lines and primary cells, and hard-to-detect mycoplasma contamination.

Key Points:

  • Cell viability/proliferation can be measured quickly and easily with a number of assays, including the ATCC MTT and XTT proliferation kits, as well as the new Reliablue™ cell viability regent.
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations with choice of media and reagents must be taken.
  • Mycoplasma contamination can cause chronic physiological problems with cultured cells and are hard to detect visually, but can be easily assessed with Mycoplasma Detection Kits and/or services, provided by ATCC.

Introduction to ATCC® Microbiome Standards

An End-To-End Solution for the Standardization of Microbiome Research

9/7/2017

Abstract:

The complexities involved in 16S rRNA and shotgun metagenomic analysis methods pose significant challenges for microbiome research. Significant biases can be introduced during PCR amplification, library preparation, sequencing, and analysis. One of the primary obstacles in assay standardization is the limited availability of reference materials. To support this need, ATCC has developed microbiome reference standards from fully sequenced and characterized ATCC strains. To further enhance the use of these standards, ATCC has collaborated with One Codex to develop an easy-to-use data analysis module that enables researchers to analyze both shotgun and 16S rRNA data quickly and accurately while eliminating the biases associated with data analysis. In this webinar, we will discuss the need for standards in microbiome research, the development and use of the ATCC® Microbiome Standards, and how to analyze these standards using the One Codex data analysis platform.

Key Points:

  • One of the primary obstacles in the standardization of microbiome assays is the limited availability of reference materials
  • ATCC has developed microbiome reference standards from fully sequenced and characterized strains for use as daily run controls and in assay development
  • To eliminate bias associated with data analysis, ATCC has collaborated with One Codex to combine the power of physical laboratory standards with the leading bioinformatics platform for microbial genomics

Abstract: Culturing anaerobes in the laboratory can be challenging. Due to the copious growth requirements and the plethora of anaerobic organisms, knowing the optimal conditions for your anaerobic organism is essential. In this webinar, we will present the various methods to achieve successful growth conditions for a wide variety of anaerobes. We will consider topics such as common anaerobic gas mixtures, media selection, and obtaining anaerobic conditions in the lab. Taking into account individual laboratory constraints, we will also discuss several propagation methods available today. Using specific examples from the ATCC collection, we will demonstrate these techniques.

Key Points:

  • There are numerous types of anaerobic bacteria with various atmospheric and nutritional requirements from clinical gut and wound isolates to environmental extremophiles.
  • Creating and maintaining anaerobic atmospheric and nutritional conditions are critical for the successful growth of anaerobic bacteria.
  • The propagation of anaerobic bacteria has evolved throughout the years. The discovery of new isolates has brought about the need for more robust culturing technology.

Angio-Ready

An improved in vitro model of angiogenesis

6/8/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Angiogenesis is a multi-step physiological process that is involved in a large number of normal and disease state processes. In this webinar, we will introduce the Angio-Ready™ Angiogenesis Assay System, an in vitro co-culture system for measuring angiogenesis. Angio-Ready™ consists of an assay-ready mixture of an hTERT-immortalized human aortic endothelial cell line (TeloHAEC-GFP) and an hTERT-immortalized adipose-derived mesenchymal stem cell line (hTERT-MSC) in a specially formulated medium. Both cell lines have been well-characterized to verify that they retain the most important characteristic of their parental counterparts. The new co-culture system forms functional tubular structures in less than 7 days and responds appropriately in a dose dependent manner to known agonists and inhibitors of angiogenesis. Thus, Angio-Ready™ is a ready-to-use, time-saving, high-throughput model for screening drugs or biomolecules for their effect on angiogenesis in cancer, toxicology, diabetes, cardiovascular disease, wound healing, and other pathologies.

Key Points:

  • Angio-Ready™ is a ready-to-use, scalable assay system for angiogenesis
  • Live imaging is possible with this in vivo-like model consisting of fine tubular structures formed in less than 7 days
  • The formation of vascular tubules responds appropriately to agonists and inhibitors of angiogenesis

ATCC Biorepository Services℠

Processing of Biospecimens in a Controlled Environment

5/25/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Biological materials produced through scientific inquiry are often valuable assets that support important scientific breakthroughs. To ensure the safety and future availability of these resources, they should be stored within a biorepository to protect against loss, contamination, or genetic drift. In this webinar, we will discuss the importance of biological materials management and will expand on the services offered at ATCC that support the handling and storage of biological specimens

Key Points:

  • Biobanking of cell lines, microbial strains, and other biological reagents generated through scientific investigation with a biorepository ensures they will be accessible to support future scientific endeavors
  • ATCC Biorepository Services℠ delivers secure and reliable biological material management with temperature-controlled supply chain, 24/7 equipment monitoring, and on-call after-hours personnel
  • ATCC provides extensive support to customers needing cGMP compliant and non-cGMP master cell banks and working cell banks, including short- and long-term storage of small- or large-scale specimens