Elevating biological models of metastasis

In this webinar, ATCC epithelial-to-mesenchymal transition expert and Senior Biologist Diana Douglas will cover the following key points:

  • CRISPR/Cas9 genome-editing technology was applied to develop a number of RFP- or GFP-tagged reporter cell lines to study EMT and MET phenomena.
  • The EMT/MET reporter cell lines can be used to monitor cellular changes in real time or as a platform for drug screening.
  • In depth transition data were generated including morphology change, intrinsic reporter expression, marker expression, and invasion upon stimulation with EMT/MET agonists.

Use of exosomes as reference materials in biomedical research

Dr. Siddhartha Paul is an expert in the isolation and characterization of extracellular vesicles. In this presentation, Dr. Paul gives an overview of exosomes from various well-characterized ATCC cell lines and shows data indicating that these extracellular vesicles can be used as reference materials in biological research and assay development.

  • There is an unmet need for well-characterized exosome reference materials.
  • Exosomes from ATCC cells can serve as consistent, natural standards for molecular assay development or utilized to develop novel drug delivery systems.
  • ATCC experts generated characterization and application data indicating that exosomes can be implemented in multiple functional assays.

Despite the availability of existing genome sequences in public databases, the quality, completeness, authenticity, accuracy, and traceability of genomic data is inadequate; the lack of standards for genome quality exacerbates these underlying problems. To address the underlying problems, ATCC has implemented a robust next-generation sequencing and genome assembly workflow to produce reference-quality microbial genomes. In this webinar, ATCC genomics expert Andrew Frank, MS, will cover these key points:

  • Understand the standardized process and quality control criteria required for growing, extracting, sequencing, and analyzing each reference-quality genome
  • Review the metadata provided on the ATCC Genome Portal to contextualize the data as it fits with your NGS workflow
  • Explore the features of the ATCC Genome Portal as well as the genomes available