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CRISPR/Cas9 genome-editing technology is a powerful new tool for developing cell-based models with disease-relevant mutations. In this presentation, we describe how this advanced technology was used to introduce specific point mutation that confers drug resistance into the A375 melanoma line to create the MEK1 Q56P Mutant-A375 Isogenic Cell Line. The new isogenic cell line is resistant to both MEK and BRAF inhibitors and, like the current isogenic KRAS and NRAS Mutant-A375 Cell Lines, sensitive to combination therapies targeting both upstream and downstream elements of the Ras/Raf-MAPK signaling pathway, making it an ideal model for screening and evaluating novel therapeutics and combination treatments targeting multidrug-resistant melanomas.

Abstract:

Throughout the world, acute respiratory infections are responsible for millions of deaths annually and are a leading cause of mortality in children. Even if not fatal, these infections can be debilitating and often result in hospitalization. Viruses such as influenza virus, respiratory syncytial virus, and enterovirus are major contributors to this disease burden. Emerging viruses have also posed significant risks to human health, as demonstrated by the SARS coronavirus outbreak in 2003 and the recent emergence of the MERS coronavirus. This underscores the importance of effective respiratory virus diagnostic assays, vaccines, and antiviral therapies. In this presentation, we will summarize the impact of respiratory viral disease on global health initiatives; the latest efforts to control and prevent infections (e.g., the universal influenza vaccine); and the importance of authenticated viruses and derivatives in the development of diagnostic assays, vaccines, and antiviral therapeutics.

Key Points:

  • Endemic and emerging respiratory virus infections represent a significant global heath burden.
  • Understanding respiratory virus epidemiology and pathogenesis is critical for the development of antiviral therapies.
  • Authenticated viral standards and derivatives are essential tools for the development and validation of novel preventative and therapeutic techniques.

ASTMH 67th Annual Meeting 2018

Precision Medicine Starts Here

10/28/2018 — 11/1/2018

New Orleans LA

The ASTMH Annual Meeting is a five-day educational conference that draws nearly 4,500 attendees -researchers, physicians in global health and tropical and travel disease, military personnel and public health officials from around the world.

11/1/2018 — 11/3/2018

San Antonio, TX

Booth: 701

AMP 2018 brings together key researchers in every aspect of molecular diagnostics, including Interpretation & Reporting of Molecular Diagnostic Tests, Assay Development, Validation & Performance, & Translational Research.

11/3/2018 — 11/7/2018

San Diego, CA

Booth: 3326

Society for Neuroscience's 48th annual meeting, Neuroscience 2018, is the world's largest neuroscience conference for scientists and physicians devoted to understanding the brain and nervous system.