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GeneXPlus Transfection Reagent (ATCC® ACS-4004)

Product Format 1 mL
Storage Conditions -20°C
Applications Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.  Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266). A complete list of cell line-specific protocols can be found here.

This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.


GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4002) to reproducibly express a wide range of proteins at high levels.

Intended Use

This product is intended for research use only. It is not intended for any animal or human therapeutic or diagnostic use.

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Introduction GeneXPlus Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells.
Quality Control Specifications

Physical characterization of reagent components is performed using mass spectrophotometry and nuclear magnetic resonance (NMR) for identification and determination of purity. GeneXPlus is tested for performance using a luciferase reporter assay in HEK293 cells.



Physical Characterization


Visual Inspection


Functional Assay

>75 ng luciferase/well


2.5 mg/mL in 80% ethanol

*Please consult the Certificate of Analysis for lot-specific test results.

Basic Documentation
Other Documentation

Yang H, et al. Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 viaWnt/β-catenin and MAPK pathway. Organogenesis 14(1):36-45, 2018. PubMed: 29913119