Sangeeta Kumari, MS, is an expert at using CRISPR/Cas9 genome-editing technology for creating advanced cell-based models. Watch the presentation to learn how this cutting edge technology was used to create epithelial-mesenchymal transition reporter cell lines.
- ATCC scientists used CRISPR/Cas9 to engineer an E-cadherin EmGFP (ECAD-EmGFP) reporter knock-in into a breast epithelial cell line.
- The integrity of the ECAD-EmGFP knock-in was verified at the genomic, mRNA, and protein level.
- This advanced cancer model can be used to track EMT status of cells in vitro by monitoring GFP expression.