Webinars

Join top virologists Adria Allen, Alexander Piccirillo, and Megan Yockey for an in-depth look at viral propagation strategies used at ATCC. This webinar will cover these takeaways:

• ATCC cultures viruses with several basic propagation types (e.g., lytic, cell-associated, persistently infected cells, egg propagation).  
• Multiple tools and processes are used to optimize protocols, including multiplicity of infection (MOI) optimization. 
• ATCC authenticates and tests each virus to make sure we are distributing the highest quality products to our customers. 
• ATCC uses numerous strategies for troubleshooting (e.g., mycoplasma cleanup, failure to grow, adaptation to new host cells).

In this webinar Diversigen, Inc. scientist Dr. Tasha Santiago-Rodrigues will discuss the field of viral metagenomics, focusing on the following key points:

• Implementation of viral standards and controls can help determine biases that could be potentially introduced at different stages of a viral metagenomics pipeline.
• Identification of biases in a viral metagenomics study provides the opportunity to troubleshoot and benchmark reagents and analysis tools. 
• Viral standards may evolve to mimic simple and more complex viral communities in diverse environments.

Steve Budd, MBA, has extensive experience with culturing hard-to-grow cell lines and primary cells. Watch this webinar to hear this ATCC expert cover the following key points:

• There are many cell models; choosing the correct one for your workflow will add efficiency and productivity to your work.
• When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken.
• Using misidentified or cross-contaminated cell lines in experiments can invalidate experimental results; therefore, authenticating cell lines should be part of your cell culture workflow.
• Mycoplasma infection can chronically affect the well-being of cells in culture without being detected by visual observation; multiple screening methods can be used to identify contamination.
• Tools such as proliferation assays and cryo-containers can aid the maintenance of cell health.

In this webinar, ATCC experts Marco Riojas and Andrew Frank discuss the critical role that ATCC and BEI Resources play in providing front-line COVID-19 researchers with the high-quality authenticated biomaterials necessary to counter the current global threat and our own research efforts in the genomic analysis of SARS-CoV-2 and the subsequent publication of our genomic data on the ATCC Genome Portal. Watch the webinar to hear:

• A description of ATCC and BEI Resources' wide range of SARS-CoV-2 standard material  
• A study depicting how we sequence SARS-CoV-2 isolates for ATCC and BEI Resources
• A recap of our efforts to reduce genomic variability in cultured SARS-CoV-2

In this webinar, ATCC biologist Brian Della Fera will present THP-1 luciferase reporter cell lines, a new tool for the development of immunomodulatory drugs and biologics. Attend this webinar to discover:

• THP-1 cell line is a commonly used surrogate model for in vitro human primary monocytes
• This luciferase reporter system provides a straightforward, robust, and sensitive means to measure biological processes through in vitro bioluminescence measurements
• THP-1 luciferase reporter cell lines allow broad application in drug development, cell signaling pathway research, and as a safety evaluation tool for new chemicals and drugs

In this webinar, ATCC experts Kevin Grady and April Jeffrey will showcase how our laboratories and biorepository can provide customized solutions for organizations that choose to outsource critical processes. Learn:

• The importance of cell authentication to support reliable and relevant research.
• How ATCC scientists can use their expertise to engineer the cell modifications you need to advance your research.
• How ATCC can produce, scale-up, store, and distribute your biomaterials under cGMP compliant conditions.

Sangeeta Kumari, MS, is an expert at using CRISPR/Cas9 genome-editing technology for creating advanced cell-based models. Watch the presentation to learn how this cutting edge technology was used to create epithelial-mesenchymal transition reporter cell lines.

• ATCC scientists used CRISPR/Cas9 to engineer an E-cadherin EmGFP (ECAD-EmGFP) reporter knock-in into a breast epithelial cell line.
• The integrity of the ECAD-EmGFP knock-in was verified at the genomic, mRNA, and protein level.
• This advanced cancer model can be used to track EMT status of cells in vitro by monitoring GFP expression.

In this webinar, ATCC bacteria propagation expert Nancy Krueger will cover various methods to achieve the growth of a wide variety of bacteria, focusing on these key takeaways:

• Specific growth requirements should be tailored to ensure the successful propagation of individual bacteria strains. Different atmospheres, temperature ranges and other growth conditions will be discussed.
• New bacterial isolates may present unique growth issues.
• A rational approach to troubleshooting can ensure the resolution of poor growth.

In this webinar, ATCC expert Kathleen Glaser will discuss protistology, presenting these key takeaways:

• What defines a protist, and why these organisms are relevant not only to scientists but also to the general community.
• An in-depth look at some of the best practices for propagating, preserving, and authenticating protists.
• An examination of the special techniques that have been adapted at ATCC for the evaluation and cultivation of difficult-to-grow protists.

Join top virologists Adria Allen, Alexander Piccirillo, and Megan Yockey for an in-depth look at viral propagation strategies used at ATCC. This webinar will cover these takeaways:

• ATCC cultures viruses with several basic propagation types (e.g., lytic, cell-associated, persistently infected cells, egg propagation).  
• Multiple tools and processes are used to optimize protocols, including multiplicity of infection (MOI) optimization. 
• ATCC authenticates and tests each virus to make sure we are distributing the highest quality products to our customers. 
• ATCC uses numerous strategies for troubleshooting (e.g., mycoplasma cleanup, failure to grow, adaptation to new host cells).