HCM-CSHL-0061-C18 (ATCC® PDM-5)

Organism: Homo sapiens, human  /  Tissue: sigmoid colon  /  Disease: primary adenocarcinoma

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Organism Homo sapiens, human
Tissue sigmoid colon
Product Format frozen 1.0 mL
Morphology organoid
Culture Properties embedded 3D culture
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease primary adenocarcinoma
Age See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://portal.hcmi.cancercollaboratory.org/

Gender female
Ethnicity See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://portal.hcmi.cancercollaboratory.org/

Applications Research purposes, compound screening
Storage Conditions liquid nitrogen vapor phase
Images
Clinical Data ICD-10-CM code: C18, malignant neoplasm of colon
https://portal.hcmi.cancercollaboratory.org/

Comments Next-generation cancer model from the Human Cancer Models Initiate (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data.

https://ocg.cancer.gov/programs/hcmi/resources
https://portal.gdc.cancer.gov/  
https://portal.hcmi.cancercollaboratory.org/

Complete Growth Medium

To prepare the complete medium for this organoid please refer to the Organoid Media Formulation #1.

Subculturing

Initiating culture from frozen vials: For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids.

Seeding density: 0.25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate. Alternatively, split at 1:2-1:4 every 7-10 days. For example, collect organoids from 100 µL of extracellular matrix (ECM) from a single well of a 6-well plate and re-seed into 2-4 wells of a 6-well plate in 100 µL ECM per well. 

Media renewal: Perform a complete medium change every 2-3 days. Include 10 µM ROCK Inhibitor Y-27632 (ATCC ACS-3030) in medium for the first 2-3 days following subculture. 

For a brief overview of the subculture and expansion of organoids see our quickstart guide Subculture and Expansion of Human Organoids Protocol.

For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology

Cryopreservation

We recommend cryopreserving this model in ATCC Stem Cell Freezing Media (ATCC ACS-6000). For a brief overview of the cryopreservation procedure for organoids see our quickstart guide Organoid Cryopreservation Protocol.

For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology.

Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial ≥ 1 x 106
Volume 1.0 mL
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viral Testing Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected
Name of Depositor Cold Spring Harbor Laboratory
Year of Origin 2017
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQ's
  1. HCMI - Human Cancer Models Initiate
    HCMI stands for the Human Cancer Models Initiate and is an international consortium with the goal of generated novel patient derived cancer models using advanced culture methods and supported wit...
    Date Updated: 1/14/2019
  2. Organoid media availability
    Currently there are no commercially available sources of organoid media that is suitable for culture of ATCC’s human organoids.
    Date Updated: 1/14/2019
  3. Extracellular matrix for organoid cultures
    We recommend ATCC CellMatrix (ACS-3035) which has been tested to support human organoid cultures and used in the manufacturing of ATCC organoids.
    Date Updated: 1/14/2019
  4. Organoid media
    Refer to the model specific media preparation instructions. We do not recommend using alternate growth media. Any deviations from the indicated formulation may impact organoid growth rate, morpho...
    Date Updated: 1/14/2019
  5. Organoid passages
    Yes. Similar to cell lines organoid models can be propagated and expanded in culture. See the associated propagation protocol linked to from the ATCC product page for a particular organoid model.
    Date Updated: 1/14/2019
  6. ATCC organoid model sequencing data
    Sequencing data can be found here:
    Date Updated: 1/14/2019
  7. Organoids
    Organoids are three-dimensional microtissues that grow embedded within an extracellular matrix. We recommend the following review article for an overview of organoid culture: https://doi.org/10.1...
    Date Updated: 1/14/2019
  8. Organoid cryopreservation and recovery
    Yes. Similar to cell lines organoid models can be cryopreserved and recovered. See the associated cryopreservation protocol linked to from the ATCC product page for a particular organoid model. W...
    Date Updated: 1/14/2019
  9. ATCC organoids in 2D
    We do not recommend propagating organoid models as 2D cultures or in liquid suspension.
    Date Updated: 1/14/2019
  10. ATCC organoids species
    All of ATCC’s organoid models are derived from human tissue.
    Date Updated: 1/14/2019
  11. ATCC organoids protocol
    We recommend the following publication for more detailed protocols on human organoid culture including propagation and cryopreservation: https://currentprotocols.onlinelibrary.wiley.com/doi/10.10...
    Date Updated: 1/14/2019
  12. ATCC organoids tissues
    We are continually adding new organoid models from different types of cancer. See the available models on the ATCC website at
    Date Updated: 1/14/2019
Restrictions

This material is only distributed under the additional terms of a fully signed HUB Addendum. Prior to shipment, all customers must sign and return the HUB Addendum.