HCM-BROD-0029-C71 (ATCC® PDM-22)

Organism: Homo sapiens, human  /  Tissue: brain  /  Disease: recurrent glioblastoma

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue brain
Product Format frozen 1.0 mL
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease recurrent glioblastoma
Age See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0029-C71

Gender male
Ethnicity See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0029-C71

Applications Basic research, compound screening
Storage Conditions liquid nitrogen vapor phase
Images
Clinical Data

ICD-10-CM code: C71, malignant neoplasm of cerebrum; Recurrent glioblastoma

See associated clinical data for patient profile information, if available.
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0029-C71

Comments Next-generation cancer model from the Human Cancer Models Initiate (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data.

https://ocg.cancer.gov/programs/hcmi/resources
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-BROD-0029-C71

Complete Growth Medium

NeuroCult NS-A Basal Medium (StemCell Technologies #05750) with NS-A Proliferation Supplement (StemCell Technologies #05754) + 20 ng/mL EGF (StemCell Technologies #78003.1) + 20 ng/mL bFGF (Peprotech #AF-100-15) + 2 µg/mL Heparin (StemCell Technologies #07980).

Prepare media according to the manufacturer’s instructions: Stem Cell Technologies Catalog #5751

Subculturing
Important: use Ultra Low Attachment (ULA) flasks/plates when culturing this model such as Corning #3814.

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5-5 X 105 cells/mL and maintain between below 1 X 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). Large neurospheres should be mechanically dissociated to small fragments by repeated pipetting. Do not dissociate to single cells.
Cryopreservation Complete growth media containing 10% DMSO (ATCC 4-X).
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial ≥ 1 x 106 cells
Volume 1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 12,13
D5S818: 10,12
D7S820: 7,10
THO1: 9,9.3
TPOX: 8
vWA: 15,19
Sterility Tests Bacteria, yeast and fungi: No growth
Mycoplasma: No growth
Viral Testing Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected
Name of Depositor Broad Institute
Year of Origin 2018
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation