hTERT TIGKs (ATCC® CRL-3397)

Organism: Homo sapiens, human  /  Tissue: gingiva epithelium  / 

Permits and Restrictions

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Organism Homo sapiens, human
Tissue gingiva epithelium
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age unknown
Gender male
Ethnicity unknown
Applications Model system to investigate the interaction between periodontal pathogens and the epithelium during the initial stages of the periodontal disease process.
Images CRL-3397
Comments Introduction of hTERT into bmi1-transduced primary gingival epithelial cells by sequential transfection.
Complete Growth Medium

The base medium for this cell is Dermal Cell Basal Medium (485 mL; ATCC PCS-200-030). To make the complete medium add the components of the Keratinocyte Growth Kit (ATCC PCS-200-040)

Contents of Keratinocyte Growth Kit 
  • rh Insulin, 0.5 mL (5 µg/mL final concentration) 
  • Bovine Pituitary Extract (BPE), 2.0 mL (0.4%)
  • L-Glutamine, 15.0 mL (6 mM final concentration) 
  • Epinephrine, 0.5 mL (1.0 µM final concentration) 
  • Apo-Transferrin, 0.5 mL (5.0 µ/mL final concentration) 
  • Hydrocortisone Hemisuccinate, 0.5 mL (100 ng/mL final concentration
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    Cultures can be established between 1.0 x 104 and 3.0 x 104 viable cells/cm2.
  6. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 5.0 X 103 and 9.0 X 104 cell/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Culture medium + 5% DMSO (ATCC 4-X)
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial Approximately 2.0 to 3.0 x 106 cells
Volume 1.0 mL
STR Profile
Amelogenin: X,Y
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11,13
D5S818: 9,12
D7S820: 10,11
THO1: 6,7
TPOX: 8,11
vWA: 17,18
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viral Testing Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected
Functional Tests Cytokeratins 13, 14, and 19 expressed in TIGKs. Transcripts for TLRs 1 through 6 are readily detected in TIGKs.
Population Doubling Time Per the depositor, 1 week
Name of Depositor University of Louisville
Year of Origin 2012
References

Moffatt-Jauregui CE, et al. Establishment and characterization of a telomerase immortalized human gingival epithelial cell line. J Periodontal Res 48(6): 713-721, 2013. PubMed: 23441958

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

For commercial accounts, this cell line is only distributed under the terms of a fully signed and executed ATCC® Material Transfer Agreement and Addendum. If the commercial account is screening per completed Addendum, the recipient will be required to pay a Screening Fee (ATCC® ACS-2103F™).

Screening Use is defined as use of Biological Material in small molecule and biologic drug discovery, including initial target identification and validation, assay development, high throughput screening, hit identification, lead optimization, and selection of candidates for clinical development.

If the commercial account is not screening per the completed Addendum, the recipient will not be required to pay a Screening Fee.

References

Moffatt-Jauregui CE, et al. Establishment and characterization of a telomerase immortalized human gingival epithelial cell line. J Periodontal Res 48(6): 713-721, 2013. PubMed: 23441958