Keeping Cells Happy

Topics in Cell Health Maintenance and Viability

9/13/2018


Abstract

Good practices are vital for safeguarding the health of cells in long-term culture. Cell health, maintenance, and viability cover a wide range of topics, from ensuring that cells are healthy in culture to having confidence that the cells are characterized correctly. This presentation will focus on topics such as cell viability assays that measure cell growth, authentication of cell lines through short tandem repeat (STR) profiling, mycoplasma testing, aseptic technique, and cryopreservation. From services to techniques, we will discuss ATCC’s role in ensuring the health and maintenance of cells in culture.

Key Points

  • Mycoplasma infection can chronically affect the well-being of cells in culture without causing obvious symptoms. Screening is required to determine if cell cultures are infected.
  • STR profiling detects misidentification or cross-contamination of human cell lines.
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken.
  • Proliferation kits and reagents such as XTT, MTT, and Reliablue™ are great tools for measuring cell health and growth.

Presenter

Steve Budd

Steven Budd, M.S., M.B.A.,
Product Line Business Specialist, ATCC

Steven Budd is a Product Line Business Specialist that manages the cell culture reagents at ATCC. He has 4 years of experience in the product management of scientific tools. Before that, he gained 4 years of experience in biomedical research and cell culture as a research specialist at the University of North Carolina at Chapel Hill. Mr. Budd has a M.S. in Biology from the University of North Carolina at Wilmington and an M.B.A. from North Carolina State University.